CelLytic™ MT Cell Lysis Reagent

Protein isolation Tissue - Mouse liver tissue

Experiment
Protein isolation Tissue - Mouse liver tissue
Product
CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
- Cut tissue is resuspended in 3 volumes of CelLytic Buffer plus 10% protease inhibitor mixture and Halt phosphatase inhibitor mixture and incubated on ice for 30.

- After the the livers are lysed using a Qiagen Tissue Lyser LT and then centrifuged at 100,000 × g at 4 °C.

Publication protocol

Mouse tissues were flash frozen in liquid nitrogen during harvesting and stored at −80 °C. For gel electrophoresis, 50–100 mg of cut tissue was then resuspended in 3 volumes of CelLytic Buffer (Sigma C3228) plus 10% protease inhibitor mixture (P2714-1BTL; Sigma) and Halt phosphatase inhibitor mixture (PI78420; Fisher), then incubated on ice for 30 min followed by lysing the livers using a Qiagen Tissue Lyser LT (Qiagen), and then centrifuged at 100,000 × g at 4 °C.

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Discussion

Discussion

5 years ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Mouse liver tissue using CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich.

Paper title
Biliverdin Reductase A Attenuates Hepatic Steatosis by Inhibition of Glycogen Synthase Kinase (GSK) 3β Phosphorylation of Serine 73 of Peroxisome Proliferator-activated Receptor (PPAR) α
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ MT Cell Lysis Reagent below.

Download PDF Download manufacturer protocol

Videos

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